This 3-day workshop is designed for those with little or no experience in molecular biology. We will cover the basics of nucleic acids, restriction enzyme use, DNA electrophoresis, purification of DNA fragments from agarose gels, preparation of plasmid vectors for subcloning and features and uses of plasmid cloning vectors, DNA ligation reactions, transformation of chemically competent E. coli, screening of recombinant clones, the isolation and analysis of recombinant E. coli clones, and finally an overview of cDNA library construction.

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