Studying kidney physiology is essential to increase our understanding and improve the quality of life and prognosis of patients with renal diseases. Organoid cultures have been developed that enable in vitro studies of donor-specific tissue in a controlled three-dimensional (3D) environment. Organoids are 3D multicellular constructs that recapitulate structural and functional characteristics of their organ of origin. Using a modified protocol originally developed for the expansion of adult intestinal Lgr5+ stem cells1, Schutgens et al. recently established kidney tubular epithelial organoids (tubuloids) derived from adult kidney tissue and urine2.